Peptide inhibitors of Macrobrachium rosenbergii nodavirus
نویسندگان
چکیده
منابع مشابه
Tracking the virus-like particles of Macrobrachium rosenbergii nodavirus in insect cells
Macrobrachium rosenbergii nodavirus (MrNv) poses a major threat to the prawn industry. Currently, no effective vaccine and treatment are available to prevent the spread of MrNv. Its infection mechanism and localisation in a host cell are also not well characterised. The MrNv capsid protein (MrNvc) produced in Escherichia coli self-assembled into virus-like particles (VLPs) resembling the native...
متن کاملProduction of monoclonal antibodies specific to Macrobrachium rosenbergii nodavirus using recombinant capsid protein.
The gene encoding the capsid protein of Macrobrachium rosenbergii nodavirus (MrNV) was cloned into pGEX-6P-1 expression vector and then transformed into the Escherichia coli strain BL21. After induction, capsid protein-glutathione-S-transferase (GST-MrNV; 64 kDa) was produced. The recombinant protein was separated using SDS-PAGE, excised from the gel, electro-eluted and then used for immunizati...
متن کاملMacrobrachium rosenbergii nodavirus infection in a giant freshwater prawn hatchery in Indonesia
A pathogen of giant freshwater prawn, Macrobrachium rosenbergii, was recently recorded in a hatchery in Yogyakarta. The clinical symptom in post-larvae (PL) was a whitish appearance of the muscles in the tail. Histological examination revealed myonecrosis with massive infiltration of myonuclei and hemocytes. RT-PCR products of 850 bp were obtained when using RNA from diseased PL as a template. ...
متن کاملMonoclonal antibodies against extra small virus show that it co-localizes with Macrobrachium rosenbergii nodavirus.
The capsid protein (CP) gene of extra small virus (XSV) expressed in Escherichia coli as a 42 kDa glutathione S-transferase (GST)-fusion protein (GST-XCP) or a 20 kDa His6-fusion protein (His6-XCP) were purified by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), combined, and used to immunize Swiss mice to produce monoclonal antibodies (MAbs). Using dot blot, Western blot,...
متن کاملAntimicrobial peptides from the hemolymph of the prawn Macrobrachium rosenbergii
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ژورنال
عنوان ژورنال: Journal of General Virology
سال: 2018
ISSN: 0022-1317,1465-2099
DOI: 10.1099/jgv.0.001116